Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Flow cytometry antibodies used.

Article Snippet: CD3-VB , Miltenyi , 130-133-133 , BW264/56 , .

Techniques: Flow Cytometry, In Vivo, In Vitro

Journal: Cancers

Article Title: Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation

doi: 10.3390/cancers13081845

Figure Lengend Snippet: Murlentamab opsonization of SKOV3-R2 + activates an effective anti-tumor T cell immune response. SKOV3-R2 + ovarian tumor cells were labeled with different 3C23K antibodies (3C23K-FcKO control, 3C23K-CHO normally fucosylated or murlentamab the low fucosylated form) and cultured in the presence of human monocyte-derived macrophages from healthy donors unstimulated (M0) or stimulated with M-CSF and IL-10 (TAMs). After 3 days of co-culture, activated T cells coming from the same healthy donor were added in the culture well for 4 more days. ( A ) The CD4 + Th1/Th2 polarization profile, ( B ) the proportion of CD3 + CD4 + CD25 + regulatory T cells and ( C ) the activation of T CD8 + cells were determined by flow cytometry after four days of co-culture. Data shown (boxplots) are the results from two different experiments (performed with two different healthy donors). * p < 0.05; ** p < 0.01; *** p < 0.001. p values were determined using one-way ANOVA analysis followed by Tukey’s multiple comparisons test.

Article Snippet: CD3-VB , Miltenyi , 130-133-133 , BW264/56 , .

Techniques: Labeling, Cell Culture, Derivative Assay, Co-Culture Assay, Activation Assay, Flow Cytometry

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Pre- and post-extraction OPG, RANKL, HGF, TNF-α, IL-18, MMP-9, TOS—total oxidant status, TAC—total antioxidant capacity, and HbA1c values in control and diabetes mellitus patients.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Control

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: ROC curve. Prediction of salivary markers in the determinism of HbA1c > 6.5%.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques:

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Post-extraction correlation of HbA1c with salivary markers (OPG, RANKL, OPG/RANKL, HGF, TNF-α, MMP-9, IL-18, TOS and TAC).

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Extraction

Journal: PLoS ONE

Article Title: Heading towards a dead end: The role of DND1 in germ line differentiation of human iPSCs

doi: 10.1371/journal.pone.0258427

Figure Lengend Snippet: Antibodies used for flow cytometry.

Article Snippet: NANOG , REA314 , PE , Recombinant human IgG1 , anti-human , 130-117-526 , Miltenyi Biotec.

Techniques: Cytometry, Conjugation Assay, Recombinant, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice

doi: 10.1111/jcmm.15493

Figure Lengend Snippet: Byakangelicin inhibits 4‐HNE–induced hepatocyte apoptosis by inhibiting ASK1/JNK pathway. A, Detection cytotoxicity of byakangelicin in liver stellate cell line LX2 using MTT. B and C, Different time and concentration gradients to detect 4‐HNE–induced hepatocyte HepG2 apoptosis protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3 and internal reference protein β‐tubulin expression with densitometry. D and E, Protein full‐length PARP, PARP, cleaved caspase‐3, caspase‐3, JNK, P‐JNK, ASK‐1 and P‐ASK‐1 were also detected and quantified using Western blot analyses. F, Immunofluorescence by using antibody against caspase‐3. G, Apoptosis detection by using flow cytometry. For the statistics of each panel in this figure, # P < .05, ## P < .01, ### P < .001; * P < .05, ** P < .01, *** P < .001, n = 3

Article Snippet: We selected HepG2 as human hepatocyte cell line., The HepG2 cells were cultured in DMEM (Solarbio Technology) with 10% foetal bovine serum (FBS; Excell Bio).

Techniques: Concentration Assay, Expressing, Western Blot, Immunofluorescence, Flow Cytometry

Journal: Journal of Cellular and Molecular Medicine

Article Title: Byakangelicin protects against carbon tetrachloride–induced liver injury and fibrosis in mice

doi: 10.1111/jcmm.15493

Figure Lengend Snippet: Mechanism diagram of byakangelicin attenuating the progression of liver fibrosis. byakangelicin inhibits the proliferation and activation of hepatic stellate cell by inhibiting the TGF/Smad3 signalling pathway and PDGF‐ERK, AKT and Stat3 signalling. In addition, byakangelicin could inhibit 4‐HNE–induced apoptosis and reduce death signalling through the ASK/JNK pathway in hepatocytes

Article Snippet: We selected HepG2 as human hepatocyte cell line., The HepG2 cells were cultured in DMEM (Solarbio Technology) with 10% foetal bovine serum (FBS; Excell Bio).

Techniques: Activation Assay

Journal: Journal of Clinical Medicine

Article Title: A Novel Bioengineered Functional Motor Unit Platform to Study Neuromuscular Interaction

doi: 10.3390/jcm9103238

Figure Lengend Snippet: Complete growth media for skeletal muscle cell proliferation.

Article Snippet: Recombinant human hepatocyte growth factor (HGF) from Sino Biological Inc. (Beijing, China) , 2.5 ng/mL , 10463-HNAS.

Techniques: Concentration Assay, Modification, Recombinant

Journal: American Journal of Translational Research

Article Title: Portal modulation effects of terlipressin on liver regeneration and survival in a porcine model subjected to 90% hepatectomy

doi:

Figure Lengend Snippet: Postoperative change in serum levels of interleukin (IL)-6 (A), hepatocyte growth factor (HGF) (B), and endothelin-1 (ET-1) (C) evaluated using enzyme-linked immunosorbent assay. Data are expressed as the median, with the 25-75% percentiles in boxes and the 5-95% percentiles as whiskers. *P < 0.05 vs. control group at the same time point.

Article Snippet: Interleukin 6 (IL-6), hepatocyte growth factor (HGF), and endothelin-1 (ET-1) serum levels were measured using commercially available ELISA kits (IL-6; Porcine IL-6 Quantikine ELISA Kit, P6000B, R&D systems, USA) (HGF; Pig hepatocyte growth factor, HGF ELISA Kit, CSB-E06795p, CUSABIO, China) (ET-1; Endothelin-1 Quantikine ELISA Kit, DET100, R&D systems, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Secretion of growth factors by adipose-derived stem cells (ASCs) and the formation of networks by co-culture with endothelial cells (ECs) or lymphatic endothelial cells (LECs). ( a ) The levels of vascular endothelial growth factor-C (VEGF-C), VEGF-A, hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in the medium bathing cultured ASCs were measured using enzyme-linked immunosorbent assays. HGF and VEGF-A were both secreted by ASCs, whereas the levels of secreted VEGF-C and b-FGF were much lower. ( b ) Green fluorescent protein (GFP)-expressing ECs and ASCs co-cultured at a ratio of 1:4. Networks of vascular ECs were observed, and the edges of the vascular EC network were sharp (arrows). ( c ) GFP-LECs and ASCs co-cultured at a ratio of 1:4. The LECs formed networks, but in contrast to vascular ECs, rounded/bulging structures were observed at the edges of the LEC network (arrowheads). ( d ) Merge image of ( c ) and phase-contrast image. ASCs shows random pattern with no specific character which do not form own network or support LEC network directly.

Article Snippet: The concentrations of VEGF-C, VEGF-A, HGF, and bFGF were measured by enzyme-linked immunosorbent assay (ELISA) using a Human VEGF-C ELISA Kit (P49767, RayBiotech, USA), LBIS Human VEGF ELISA Kit (631-40831, Fujifilm Wako, Japan), AuthentiKine Human HGF ELISA Kit (KE00168, Proteintech, USA) and Human bFGF ELISA Kit (P09038, RayBiotech, USA) in accordance with the manufacturers’ instructions.

Techniques: Derivative Assay, Co-Culture Assay, Cell Culture, Expressing

Journal: Neural Regeneration Research

Article Title: Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms

doi: 10.4103/1673-5374.155442

Figure Lengend Snippet: hUCMSCs express and secrete extracellular matrix and neurotrophic factors that enhance nerve regeneration. (A–C) Extracellular matrix components deposited by hUCMSCs were visualized by immunofluorescence staining. Arrows indicate positive expression. FITC was the dye. Scale bars: 200 μm. (D) Cytokine antibody array assay revealed neurotrophic factor expression. hUCMSC-1 and hUCMSC- 2 represent cultures derived from different umbilical cords. (E, F) BDNF, GDNF, HGF, NT-3, bFGF, NGF-β and VEGF protein levels were detected in hUCMSC-conditioned medium and control medium by ELISA. All data are expressed as the mean ± SD. Statistical analysis was performed using one-way analysis of variance followed by Tukey's test. ** P < 0.01, vs . control (blank) medium. hUCMSCs: Human umbilical cord-derived mesenchymal stem cells; IL-6: interleukin-6; BDNF: brain-derived neurotrophic factor; TGF-β: tumor growth factor-β; EGF: epidermal growth factor; NAP-2: neutrophil activating protein-2; NT-3: neurotrophin-3; HGF: hepatocyte growth factor; GDNF: glial-derived neurotrophic factor; VEGF: vascular endothelial growth factor; IGF-1: insulin-like growth factor-1; PDGF: platelet-derived growth factor; NT-4: neurotrophin-4; LIF: leukemia inhibitory factor; SCF: stem cell factor; bFGF: basic fibroblast growth factor; NGF-β: nerve growth factor-β; ELISA: enzyme linked immunosorbent assay.

Article Snippet: The conditioned media were analyzed using human brain-derived neurotrophic factor (BDNF), glial-derived neurotrophic factor (GDNF), hepatocyte growth factor (HGF), neurotrophin-3 (NT-3), basic fibroblast growth factor (bFGF), nerve growth factor (NGF)-β and vascular endothelial growth factor (VEGF) ELISA kits (Boster, Wuhan, China) according to the manufacturer's protocols.

Techniques: Immunofluorescence, Staining, Expressing, Ab Array, Derivative Assay, Control, Enzyme-linked Immunosorbent Assay